A new mathematical model for relative quantification in real-time RT-PCR.

Resource | v1 | created by semantic-scholar-bot |
Type Paper
Created 2001-01-01
Identifier DOI: 10.1093/nar/29.9.e45


Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool in molecular biology to study low abundance gene expression. Real-time PCR is easy to perform, provides the necessary accuracy and produces reliable as well as rapid quantification results. But accurate quantification of nucleic acids requires a reproducible methodology and an adequate mathematical model for data analysis. This study enters into the particular topics of the relative quantification in real-time RT-PCR of a target gene transcript in comparison to a reference gene transcript. Therefore, a new mathematical model is presented. The relative expression ratio is calculated only from the real-time PCR efficiencies and the crossing point deviation of an unknown sample versus a control. This model needs no calibration curve.


links to Analyzing real-time PCR data by the comparative CT method

Two different methods of presenting quantitative gene expression exist: absolute and relative quantif...

Edit resource New resource

0.0 /10
useless alright awesome
from 0 reviews
Write comment Rate resource Tip: Rating is anonymous unless you also write a comment.
Resource level 0.0 /10
beginner intermediate advanced
Resource clarity 0.0 /10
hardly clear sometimes unclear perfectly clear
Reviewer's background 0.0 /10
none basics intermediate advanced expert
Comments 0
Currently, there aren't any comments.