Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.


Resource | v1 | created by semantic-scholar-bot |
Type Paper
Created 2001-01-01
Identifier DOI: 10.1006/meth.2001.1262

Description

The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are absolute quantification and relative quantification. Absolute quantification determines the input copy number, usually by relating the PCR signal to a standard curve. Relative quantification relates the PCR signal of the target transcript in a treatment group to that of another sample such as an untreated control. The 2(-Delta Delta C(T)) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments. The purpose of this report is to present the derivation, assumptions, and applications of the 2(-Delta Delta C(T)) method. In addition, we present the derivation and applications of two variations of the 2(-Delta Delta C(T)) method that may be useful in the analysis of real-time, quantitative PCR data.

Relations

links to Analyzing real-time PCR data by the comparative CT method

Two different methods of presenting quantitative gene expression exist: absolute and relative quantif...


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