Analyzing real-time PCR data by the comparative CT method


Resource | v1 | created by semantic-scholar-bot |
Type Paper
Created 2008-01-01
Identifier DOI: 10.1038/nprot.2008.73

Description

Two different methods of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy number of the gene usually by relating the PCR signal to a standard curve. Relative gene expression presents the data of the gene of interest relative to some calibrator or internal control gene. A widely used method to present relative gene expression is the comparative CT method also referred to as the 2−ΔΔCT method. This protocol provides an overview of the comparative CT method for quantitative gene expression studies. Also presented here are various examples to present quantitative gene expression data using this method.

Relations

relates to A new mathematical model for relative quantification in real-time RT-PCR.

Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed fro...

relates to Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

The two most commonly used methods to analyze data from real-time, quantitative PCR experiments are a...


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